Hdl Direct Measure Peg

Direct method for example measures hdl cholesterol di rectly in unprocessed serum samples by using polyethylene glycol peg modified cholesterol esterase and cholesterol.

Hdl direct measure peg. When cholesterol esterase and cholesterol oxidase enzymes were modified with peg they s. Direct measurement of hdl c mass by precipitation. Direct measurement of high density lipoprotein cholesterol in serum with polyethylene. High density lipoprotein hdl is the fraction of plasma lipoprotein with a hydrated.

The test for high density lipoprotein cholesterol hdl c is used as part of a lipid panel to screen for unhealthy levels of lipids and to determine your risk of developing heart disease and help make decisions about what treatment to use if you have borderline risk intermediate risk or high risk. H okabe t irie k uekama n kayahara k miyauchi direct measurement of high density lipoprotein cholesterol in serum with. 09 14 hdl direct cholesterol page 2 4 reagent 2. Hdl cholesterol esters are converted to hdl cholesterol by peg cholesterol esterase.

The hdl cholesterol is acted upon by peg cholesterol oxidase. We have developed an automated method for measuring high density lipoprotein hdl cholesterol in serum without prior separation using polyethylene glycol peg modified enzymes and sulfated alpha cyclodextrin. Calibrations are defined for the direct hdl c assay. Components concentration detergent 2 peg cholesterol esterase 5 ku l peg cholesterol oxidase 5 ku l 4 aap 1 gr dl peroxidase 8000 u l reagent preparation reagents are ready to use liquid.

The combination of peg modified enzymes with alpha cyclodextrin sulfate provided selectivity for the determination of. Well accepted now is the inverse association between hdl cholesterol hdl c 1 and risk of developing coronary artery disease cad a relationship that persists after adjustment for other risk factors and is consistent with putative protective mechanisms the measurement of hdl c in characterizing risk for cad and managing treatment of dyslipidemia has become increasingly common in. A high density lipoprotein hdl test measures the level of good cholesterol in your blood. The other components of a lipid panel typically include total cholesterol ldl cholesterol ldl.

Peg precipitation preserves the integrity of pre β hdl particles. A protocol of the direct method for example measures hdl cholesterol directly in unprocessed serum samples by using polyethylene glycol peg modified cholesterol esterase and cholesterol.